An Iranian state-run broadcaster was apparently hacked on air Saturday, and a protest against the country's leader interrupted a news bulletin.
An image of Ali Khamenei with flames surrounding him appeared on the screen after a mask appeared on the screen.
Adalat Ali, or Ali's Justice, was the name of the group.
New unrest over Mahsa Amini's death has resulted in at least three deaths when protesters clashed with security forces.
Amini was detained by morality police in Tehran for allegedly not covering her hair properly.
Three days after her arrest, the 22-year-old Iranian Kurd died in custody. Her death has sparked an unprecedented wave of protest across the country.
During Saturday's TV news bulletin at 21:00 (17:30 GMT), images included Iran's supreme leader with a target on his head, as well as images of Amini and three other women killed in recent protests.
There was a caption that read "join us and rise up", while another said "our youths' blood is dripping off your paws".
Shortly after the interruption, the call was cut off.
In Iran, rebellions against Ayatollah Ali Khamenei are historically rare, and he wields almost complete power.
But following Amini’s death, there has been widespread open dissent.
Also on Saturday, social media videos emerged which seemed to show female students at a university in Tehran chanting “get lost” during a visit by President Ebrahim Raisi.
Earlier in the day, two people were killed in Sanandaj, including a man shot in his car after he sounded his horn in support of protesters.
A video shared online also showed a woman shot in the neck lying unconscious on the ground in Mashhad.
In Sanandaj, a police official said a man had been killed by “counter-revolutionaries”, the state-run news agency IRNA reported.
On Friday, Iran’s Forensic Medicine Organization said Amini had died from multiple organ failure caused by cerebral hypoxia — and not from blows to the head, as her family and protesters contend.
Rights groups say more than 150 people have been killed since the protests in the Islamic Republic began on Sept. 17.
Shops in several cities have shut in support of the protesters, including in Tehran’s bazaar where some set fire to a police kiosk and chased the security forces away.
The protests reaching the bazaar in Tehran will ring alarm bells with Iranian leaders who have counted the merchants as among their supporters.